Purple Biotech Presents New Data for its Novel Tri-Specific T Cell and NK Cell Engagers Antibody Platform, CAPTN-3, at the EORTC-NCI-AACR Symposium on Molecular Targets and Cancer Therapeutics
Purple Biotech presented new data for its CAPTN-3 platform at the EORTC-NCI-AACR Symposium. The platform demonstrates sustained tumor regression in triple negative breast cancer models and dose-dependent activity in non-small cell lung cancer. The lead tribody IM1240 showed 5T4-dependent cytokine release with improved safety profile through conditional activation. The platform enables development of tri-specific antibodies targeting both T cells and NK cells to fight cancer, with potential first human clinical studies by 2026. The technology showed synergistic effects in tumor suppression and demonstrated plug-and-play capabilities for targeting different cancer types.
Purple Biotech ha presentato nuovi dati per la sua piattaforma CAPTN-3 al Simposio EORTC-NCI-AACR. La piattaforma dimostra una regressione tumorale sostenuta in modelli di cancro al seno triplo negativo e un'attività dose-dipendente nel cancro polmonare non a piccole cellule. Il tribody principale IM1240 ha mostrato un rilascio di citochine dipendente da 5T4 con un profilo di sicurezza migliorato grazie all'attivazione condizionale. La piattaforma consente lo sviluppo di anticorpi tri-specifici che mirano sia alle cellule T che alle cellule NK per combattere il cancro, con potenziali studi clinici umani entro il 2026. La tecnologia ha mostrato effetti sinergici nella soppressione tumorale e ha dimostrato capacità di plug-and-play per mirare a diversi tipi di cancro.
Purple Biotech presentó nuevos datos sobre su plataforma CAPTN-3 en el Simposio EORTC-NCI-AACR. La plataforma demuestra regresión tumoral sostenida en modelos de cáncer de mama triple negativo y actividad dependiente de la dosis en cáncer de pulmón no microcítico. El tribody principal IM1240 mostró liberación de citocinas dependiente de 5T4 con un perfil de seguridad mejorado mediante activación condicional. La plataforma permite el desarrollo de anticuerpos tri-específicos que apuntan tanto a células T como a células NK para combatir el cáncer, con potenciales estudios clínicos en humanos para 2026. La tecnología mostró efectos sinérgicos en la supresión tumoral y demostró capacidades de plug-and-play para dirigirse a diferentes tipos de cáncer.
Purple Biotech는 EORTC-NCI-AACR 심포지엄에서 CAPTN-3 플랫폼에 대한 새로운 데이터를 발표했습니다. 이 플랫폼은 삼중 음성 유방암 모델에서 지속적인 종양 퇴행을 나타내며 비소세포 폐암에서 용량 의존적인 활성을 보여줍니다. 주요 트리바디 IM1240는 5T4 의존적인 사이토카인 방출을 보여주었으며 조건부 활성화를 통해 안전성을 향상시켰습니다. 이 플랫폼은 암과 싸우기 위해 T세포와 NK세포를 모두 표적하는 삼중 특이적 항체의 개발을 가능하게 하며, 2026년까지 최초의 인간 임상 연구 가능성이 있습니다. 이 기술은 종양 억제에서 상승 효과를 보여주었으며 다양한 암 유형을 표적화하기 위한 플러그 앤 플레이 기능을 입증했습니다.
Purple Biotech a présenté de nouvelles données concernant sa plateforme CAPTN-3 lors du symposium EORTC-NCI-AACR. Cette plateforme démontre une régression tumorale durable dans des modèles de cancer du sein triple négatif et une activité dépendante de la dose dans le cancer du poumon non à petites cellules. Le principal tribody IM1240 a montré une libération de cytokines dépendante de 5T4 avec un profil de sécurité amélioré grâce à une activation conditionnelle. La plateforme permet le développement d'anticorps tri-spécifiques ciblant à la fois les cellules T et les cellules NK pour combattre le cancer, avec des études cliniques humaines potentielles d'ici 2026. La technologie a montré des effets synergiques dans la suppression tumorale et a démontré des capacités de plug-and-play pour cibler différents types de cancer.
Purple Biotech präsentierte neue Daten zu ihrer CAPTN-3-Plattform beim EORTC-NCI-AACR-Symposium. Die Plattform zeigt eine nachhaltige Tumorrückbildung in Modellen von triple-negativem Brustkrebs und eine dosierungsabhängige Aktivität bei nicht-kleinzelligem Lungenkrebs. Das führende Tribody IM1240 zeigte 5T4-abhängige Zytokinfreisetzung mit einem verbesserten Sicherheitsprofil durch konditionale Aktivierung. Die Plattform ermöglicht die Entwicklung von tri-spezifischen Antikörpern, die sowohl T-Zellen als auch NK-Zellen anvisieren, um Krebs zu bekämpfen, mit potenziellen ersten klinischen Studien am Menschen bis 2026. Die Technologie zeigte synergistische Effekte bei der Tumorsuppression und wies Plug-and-Play-Funktionen zur Zielgerichteten Ansprache verschiedener Krebsarten auf.
- Demonstrated sustained tumor regression in triple negative breast cancer models
- Showed dose-dependent and synergistic effects in non-small cell lung cancer patient-derived explants
- Platform's flexibility allows targeting multiple cancer types through plug-and-play design
- Potential advancement to human clinical trials by 2026
- Clinical trials haven't started yet, indicating early development stage
- Commercialization timeline extends multiple years into the future
Insights
The CAPTN-3 platform shows promising preclinical results in cancer immunotherapy through a novel tri-specific antibody approach. The lead compound IM1240 demonstrates several key advantages:
- Dual engagement of both T cells and NK cells for enhanced tumor killing
- Selective activation in the tumor microenvironment, suggesting improved safety profile
- Sustained tumor regression in triple negative breast cancer models
- Dose-dependent activity against non-small cell lung cancer
- Controlled cytokine release dependent on target presence
While these early results are encouraging, investors should note that clinical trials are not expected to begin until 2026. The platform's versatility in targeting different cancers could attract partnership opportunities, though significant development and regulatory hurdles remain before commercialization.
The market opportunity for this technology is substantial given the growing immunotherapy space. Key investment considerations include:
- Platform technology with multiple potential applications through "plug and play" capabilities
- Differentiated mechanism targeting both innate and adaptive immunity
- Potential licensing and partnership opportunities
- Long development timeline to clinical trials
With a market cap of only
CAPTN-3 demonstrates sustained tumor regression in a triple negative breast cancer in-vivo model; A dose dependent activity and a synergistic effect of the engager arms also seen in non-small cell lung cancer patient-derived explants
Lead tribody IM1240 data demonstrated that cytokine release is 5T4-dependent and suppressed by the conditionally activated capping technology, suggesting preferred safety profile
Demonstration of additional tribodies suggests CAPTN-3 plug and play platform capability
REHOVOT, Israel, Oct. 25, 2024 (GLOBE NEWSWIRE) -- Purple Biotech Ltd. ("Purple Biotech" or "the Company") (NASDAQ/TASE: PPBT), a clinical-stage company developing first-in-class therapies that overcome tumor immune evasion and drug resistance, today announced new data regarding its tri-specific antibody platform, CAPTN-3, which were presented at the 36th European Organization for Research and Treatment of Cancer, National Cancer Institute, American Association for Cancer Research (EORTC-NCI-AACR) Symposium on Molecular Targets and Cancer Therapeutics (the “Triple Meeting”) on October 25, 2024 in Barcelona, Spain.
“CAPTN-3 is a novel technology platform allowing Purple Biotech to develop a variety of new tri-specific antibodies to address different targets on tumors and different mechanisms to treat cancer patients. Our Investigational New Drug (IND) enabling work is aimed to reach first in human clinical studies with our lead candidate IM1240 potentially by 2026,” said Gil Efron, Purple Biotech CEO. “Development of this differentiated platform allows us to potentially offer partners and patients additional antibodies to treat different cancer types.”
CAPTN-3 is a novel platform technology of conditionally activated tri-specific antibodies engaging both T cells and NK cells to target cancers expressing Tumor Associated Antigen (TAA) (αCD3xαTAAxαNKG2A) to induce a strong and selective immune response against the tumor. The addition of the NK engager aims to enhances the tumor-killing activity, providing a key differentiation for more sustained and potent anti-tumor effects. The anti-NKG2A arm also acts as a checkpoint inhibitor enabling simultaneous NK and T-cell activation. This scaffold is designed to be activated only at the tumor microenvironment (TME) to improve the safety profile and extend the therapeutic index. The capped-αCD3 is cleaved by multiple TME-specific proteases, increasing the likelihood of activation by various tumor types. To further extend the capped tribody half-life, human serum albumin is included. CAPTN-3 leverages a plug and play scaffold system providing a flexible design to easily swap and integrate various antibodies to target a wide range of diseases.
The platform’s lead compound IM1240 (aCD3xa5T4xaNKG2A) targets 5T4, a TAA expressed in a variety of solid tumors and which is correlated with advanced disease, increased invasiveness, and poor clinical outcome.
“Unleashing both innate and adaptive immune subsets against the tumor through potential engagement of both T cells and NK cells to the tumor, accompanied by NKG2A:HLA-E checkpoint blockage enabling simultaneous activation of NK cell and highly cytotoxic NKG2A+ T cell subsets, is suggested as a powerful mechanism of CAPTN-3 to enhance the anti-tumor activity, and differentiates this approach from others in the field. Our CAPTN-3 platform’s lead candidate, IM1240, was found to induce enhanced and synergistic anti-tumor activity in a clinically-relevant PDE assay of a 5T4-expressing biopsy, as compared to the T cell engager or the NK cell engager bispecific derivatives. CAPTN-3’s conditional activation mechanism, including a selective capping cleavage by TME proteases and TAA dependency, potentially improve safety and efficacy, and therefore result in a wider therapeutic window. The advantages of the cleavable capping technology, in aspects of efficacy, drug exposure, and safety are demonstrated in this poster,” stated Purple Biotech’s VP Research and Development, Dr. Hadas Reuveni. “IM1240’s target, 5T4, which associates with advanced disease, increased invasiveness and poor prognosis, is an attractive target for a variety of solid cancers.”
Purple Biotech’s poster titled “CAPTN-3: A novel platform of conditionally activated T cell and NK cell engagers”, which can be viewed HERE, presented the following:
- The lead compound, IM1240 demonstrated high affinity binding towards CD3 & NKG2A proteins and CD3 & NKG2A expressing cells, while no binding was detected using the mutated versions of the tribody, indicating specificity. The synergistic effects of the αCD3 and αNKG2A arms in suppressing 5T4+ non-small cell lung cancer (NSCLC) patient-derived explant (PDE) at 10nM were demonstrated, emphasizing CAPTN-3’s potential advantage in a clinically-relevant biological assay. A dose-dependent effect of IM1240 was shown.
- Sustained tumor regression in triple negative breast cancer humanized mice was demonstrated for the capped tribody, which was superior to the uncapped tribody. No effect was shown with the non-cleavable capped tribody whose CD3 binding function is irreversibly blocked.
- Cytotoxic effect and binding to CD3 was fully recovered following capping cleavage.
- PBMC-mediated cytotoxicity against 5T4+ cancer cells was demonstrated at picomolar EC50 while no effect was observed in 5T4- cancer cells.
- IM1240 and non-capped tribody inhibited NKG2A-HLA-E interaction in a dose-dependent manner, while NKG2A mutated tribody had no effect.
- NK cell mediated cytotoxicity against HLA-E expressing cancer cells was demonstrated, while NKG2A mutated tribody had no effect.
- A cytokine release assay from hPBMC showed a 5T4+ cancer cell dependency and was inhibited by the cap, showing superior safety profile.
- Plug and Play abilities of the platform were demonstrated through different tribodies targeting 5T4 (αCD3xα5T4xαNKG2A), EGFR (αCD3xαEGFRxαNKG2A) and NKG2D (αCD3xα5T4xαNKG2D) which demonstrated low nM and selective binding to cells overexpressing the target, and efficient PBMC and NK mediated cytotoxicity.
About Purple Biotech
Purple Biotech Ltd. (NASDAQ/TASE: PPBT) is a clinical-stage company developing first-in-class therapies that seek to overcome tumor immune evasion and drug resistance. The Company's oncology pipeline includes CM24, NT219 and IM1240. CM24 is a humanized monoclonal antibody that blocks CEACAM1, that supports tumor immune evasion and survival through multiple pathways. CEACAM1 on tumor cells, immune cells and neutrophils extracellular traps is a novel target for the treatment of multiple cancer indications. As a proof of concept of these novel pathways, the Company is advancing CM24 as a combination therapy with anti-PD-1 checkpoint inhibitors in a Phase 2 study for the treatment of pancreatic ductal adenocarcinoma (PDAC). The Company has entered into a clinical collaboration agreement with Bristol Myers Squibb for the Phase 2 clinical trials to evaluate the combination of CM24 with the PD-1 inhibitor nivolumab in addition to chemotherapy. NT219 is a dual inhibitor, novel small molecule that simultaneously targets IRS1/2 and STAT3. A Phase 1 dose escalation study was concluded as a monotherapy and in combination with cetuximab in which NT219 demonstrated anti-tumor activity in combination with cetuximab in second line patients with recurrent and/or metastatic SCCHN (R/N SCCHN). The Company is advancing CAPTN-3, a preclinical platform of conditionally-activated tri-specific antibody that engages both T cells and NK cells to induce a strong, localized immune response within the tumor microenvironment. The cleavable capping technology confines the compound's therapeutic activity to the local tumor microenvironment, and thereby potentially increases the anticipated therapeutic window in patients. The third arm specifically targets the Tumor Associated Antigen (TAA). The technology presents a novel mechanism of action by unleashing both innate and adaptive immune systems to induce an optimal anti-tumoral immune response. IM1240 is the first tri-specific antibody in development that targets 5T4 expressed in a variety of solid tumors and which is correlated with advanced disease, increased invasiveness and poor clinical outcomes. The Company's corporate headquarters are located in Rehovot, Israel. For more information, please visit https://purple-biotech.com/.
Forward-Looking Statements and Safe Harbor Statement
Certain statements in this press release that are forward-looking and not statements of historical fact are forward-looking statements within the meaning of the safe harbor provisions of the Private Securities Litigation Reform Act of 1995. Such forward-looking statements include, but are not limited to, statements that are not statements of historical fact, and may be identified by words such as "believe", "expect", "intend", "plan", "may", "should", "could", "might", "seek", "target", "will", "project", "forecast", "continue" or "anticipate" or their negatives or variations of these words or other comparable words or by the fact that these statements do not relate strictly to historical matters. You should not place undue reliance on these forward-looking statements, which are not guarantees of future performance. Forward-looking statements reflect our current views, expectations, beliefs or intentions with respect to future events, and are subject to a number of assumptions, involve known and unknown risks, many of which are beyond our control, as well as uncertainties and other factors that may cause our actual results, performance or achievements to be significantly different from any future results, performance or achievements expressed or implied by the forward-looking statements. Important factors that could cause or contribute to such differences include, among others, risks relating to: the plans, strategies and objectives of management for future operations; product development for NT219, CM24 and IM1240; the process by which such early stage therapeutic candidates could potentially lead to an approved drug product is long and subject to highly significant risks, particularly with respect to a joint development collaboration; the fact that drug development and commercialization involves a lengthy and expensive process with uncertain outcomes; our ability to successfully develop and commercialize our pharmaceutical products; the expense, length, progress and results of any clinical trials; the impact of any changes in regulation and legislation that could affect the pharmaceutical industry; the difficulty in receiving the regulatory approvals necessary in order to commercialize our products; the difficulty of predicting actions of the U.S. Food and Drug Administration or any other applicable regulator of pharmaceutical products; the regulatory environment and changes in the health policies and regimes in the countries in which we operate; the uncertainty surrounding the actual market reception to our pharmaceutical products once cleared for marketing in a particular market; the introduction of competing products; patents obtained by competitors; dependence on the effectiveness of our patents and other protections for innovative products; our ability to obtain, maintain and defend issued patents; the commencement of any patent interference or infringement action against our patents, and our ability to prevail, obtain a favorable decision or recover damages in any such action; and the exposure to litigation, including patent litigation, and/or regulatory actions, and other factors that are discussed in our Annual Report on Form 20-F for the year ended December 31, 2023 and in our other filings with the U.S. Securities and Exchange Commission ("SEC"), including our cautionary discussion of risks and uncertainties under "Risk Factors" in our Registration Statements and Annual Reports. These are factors that we believe could cause our actual results to differ materially from expected results. Other factors besides those we have listed could also adversely affect us. Any forward-looking statement in this press release speaks only as of the date which it is made. We disclaim any intention or obligation to publicly update or revise any forward-looking statement or other information contained herein, whether as a result of new information, future events or otherwise, except as required by applicable law. You are advised, however, to consult any additional disclosures we make in our reports to the SEC, which are available on the SEC's website, https://www.sec.gov.
Investors relations Contact:
IR@purple-biotech.com
FAQ
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